PHYSICAL STUDIES OF ISOLATED EUCARYOTIC NUCLEI
نویسندگان
چکیده
منابع مشابه
Physical Studies of Isolated Eucaryotic Nuclei
The degree of chromatin condensation in isolated rat liver nuclei and chicken erythrocyte nuclei was studied by phase-contrast microscopy as a function of solvent pH, K(+) and Mg(++) concentrations Data were represented as "phase" maps, and standard solvent conditions selected that reproducibly yield granular, slightly granular, and homogeneous nuclei Nuclei in these various states were examine...
متن کاملGlutaraldehyde Fixation of Isolated Eucaryotic Nuclei
Isolated chicken erythrocyte nuclei have been incubated with dilute concentrations of the bifunctional cross-linking agent glutaraldehyde (0-20 mM) in order to stabilize histone-histone interactions within the native nucleus. The kinetics of the disappearance of acid-soluble histones, free amino groups, and of individual histones have been observed to be pseudo first-order. Apparent first-order...
متن کاملStudies on Isolated Nuclei
This paper describes the subfractionation of nuclei isolated from guinea pig liver by the procedure presented in the first article of the series (8). Centrifugation in a density gradient system of nuclear fractions disrupted by sonication permits the isolation of the following subfractions: (a) a nucleolar subfraction which consists mainly of nucleoli surrounded by a variable amount of nucleolu...
متن کاملGLUTARALDEHYDE FIXATION OF ISOLATED EUCARYOTIC NUCLEI Evidence for Histone-Histone Proximity
Isolated chicken erythrocyte nuclei have been incubated with dilute concentrations of the bifunctional cross-linking agent glutaraldehyde (0-20 mM) in order to stabilize histonehistone interactions within the native nucleus . The kinetics of the disappearance of acidsoluble histones, free amino groups, and of individual histones have been observed to be pseudo first-order . Apparent first-order...
متن کاملSome Enzymes of Isolated Nuclei
THE COMPOSITION OF ISOLATED NUCLEI AND CELL PREPARATIONS FROM TISSUES OF CALF, BEEF, HORSE, AND FOWL WAS STUDIED WITH RESPECT TO THE FOLLOWING COMPONENTS: 1. Liver and kidney arginase, catalase, and uricase; pancreatic lipase and amylase; cardiac muscle myoglobin; erythrocyte hemoglobin; intestinal alkaline phospharase. These are referred to as "special" components in view of their characterist...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1972
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.53.3.715